[Download] "Comparative Efficacy of Pcr-Based Restriction Fragment Length Polymorphism (RFLP) & Multiplex PCR for Glycoprotein B (Gb) Genotyping of Human Cytomegalovirus (Polymerase Chain Reaction) (Report)" by Indian Journal of Medical Research # eBook PDF Kindle ePub Free

Comparative Efficacy of Pcr-Based Restriction Fragment Length Polymorphism (RFLP) & Multiplex PCR for Glycoprotein B (Gb) Genotyping of Human Cytomegalovirus (Polymerase Chain Reaction) (Report)

eBook details

Title: Comparative Efficacy of Pcr-Based Restriction Fragment Length Polymorphism (RFLP) & Multiplex PCR for Glycoprotein B (Gb) Genotyping of Human Cytomegalovirus (Polymerase Chain Reaction) (Report)
Author : Indian Journal of Medical Research
Release Date : January 01, 2007
Genre: Life Sciences,Books,Science & Nature,
Pages : * pages
Size : 231 KB

Description

Background & objectives: Glycoprotein B (gB), involved in ceil-to-cell transmission of human cytomegalovirus (HCMV), is a critical factor in tissue tropism and viral pathogenesis. The aim of the present study was to compare the efficiency of PCR-based RFLP and multiplex nested PCR for gB gene of HCMV to determine their genotype in clinical specimens from patients with HCMV. Methods: The PCR based RFLP and the multiplex nested PCR were applied on standard strain of HCMV AD169, 4 clinical HCMV isolates and 70 clinical specimens positive for HCMV by pp65 antigenaemia assay or nested PCR for mtr II region or both.